A conditional silencing suppression system for transient expression
نویسندگان
چکیده
منابع مشابه
Silencing on the spot. Induction and suppression of RNA silencing in the Agrobacterium-mediated transient expression system.
The Agrobacterium-mediated transient expression assay in intact tissues has emerged as a rapid and useful method to analyze genes and gene products in plants. In many cases, high levels of active protein can be produced without the need to produce transgenic plants. In this study, a series of tools were developed to enable strong or weak induction of RNA silencing and to suppress RNA silencing ...
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Existing conditional expression systems can be classified in two major categories that are based either on the induction or on the de-repression of transcription. The system described here combines both mechanisms, since a unique transcription factor can be shifted from a repression to a stimulation activity by simply changing its ligand. The resulting advantage of this system is the complete a...
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BACKGROUND Conditional expression vectors have become a valuable research tool to avoid artefacts that may result from traditional gene expression studies. However, most systems require multiple plasmids that must be independently engineered into the target system, resulting in experimental delay and an increased potential for selection of a cell subpopulation that differs significantly from th...
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Transient silencing made permanent A ll cancers have genes that are permanently silenced by DNA methylation. Yeshayahu Schlesinger, Howard Cedar (The Hebrew University, Israel), and colleagues, and Martin Widschwendter (UCL, London, UK), Peter Laird (USC, Los Angeles, CA), and colleagues inspect these irreversibly silenced genes. In normal tissues, they fi nd, these genes carry transient repres...
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We have established a rapid and sensitive transient expression system for Dictyostelium discoideum. We constructed a gene fusion containing the promoter from the Dictyostelium Actin 15 gene fused to the firefly luciferase gene. The enzymatic activity of this gene fusion, expressed at very high levels in stable transformants, was measured to determine optimum conditions for transient expression ...
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ژورنال
عنوان ژورنال: Scientific Reports
سال: 2018
ISSN: 2045-2322
DOI: 10.1038/s41598-018-27778-3